Figure 3

Rheological and mechanical characterization of the click chemistry hydrogel demonstrates properties suitable for 3D bioprinting. The material's shear-thinning behavior and rapid recovery enable precise deposition of cell-laden constructs.

Figure 5

VEGF165-overexpressing endothelial cells encapsulated within the hydrogel are assessed for viability and vascular network formation. The growth factor expression aims to promote angiogenesis, addressing the vascular injury central to non-healing diabetic wounds.

Figure 6

In vitro wound healing assays using the bioprinted hydrogel constructs demonstrate enhanced cell migration and proliferation. The results suggest that the all-peptide platform supports the cellular processes required for tissue regeneration.

Figure 7

Gene expression or protein analysis from cells cultured in the hydrogel platform reveals upregulation of angiogenic and wound healing markers. The molecular data support the functional benefits observed in cell migration and proliferation assays.

Figure 8

In vivo evaluation of the bioprinted hydrogel in a diabetic wound model shows wound closure progression over time. The cell-laden constructs demonstrate accelerated healing compared to control treatments in the high-glucose wound environment.

Figure 9

Histological analysis of healed wound tissue reveals improved tissue architecture and vascularization in the hydrogel-treated group. Hematoxylin and eosin staining shows more organized collagen deposition and reduced inflammatory infiltrate.